This article reviews how the Czech Republic is implementing sustainable urban development (SUD) strategies in 2014-20 as a part of ERDF Regulation Article 7 requirements, through the use of Integrated Territorial Investments (ITIs). The article provides an overview of preparation of these instruments in the Czech Republic and how they are being implemented. The specific case of the Brno metropolitan area is used to provide a detailed example at local level of the operationalisation of SUD and ITI instruments, along with policy recommendations gathered from implementation experience so far.